This species is known from the departments of Antioquia, Caldas (type locality: Samaná), and Tolima on the eastern flank of the Cordillera Central, in Colombia, from 1,180-1,500m asl.
Habitat and Ecology
It is found mostly inside mature forests, but also occurs in areas near the forest edge (though it has not been recorded from anthropogenic habitats). It is nocturnal, and occurs on vegetation alongside small streams. It breeds via direct development.
It is not a common species.
Habitat loss caused by logging and agricultural development (livestock, illegal crops) is the major threat to the relic patch of forest in which this species occurs. Some other species of Eleutherodactylus that are associated with streams have undergone dramatic declines and disappearances, possibly due to chytridiomycosis, so the status of this species should be monitored carefully.
This species' range includes Florencia Forest, an area in the process of being declared a natural reserve.
Fernando Castro, Maria Isabel Herrera, John Lynch 2004. Pristimantis penelopus. In: IUCN 2014