© 2011 Michael Graziano (1 of 116)
Distribution and Habitat
Country distribution from AmphibiaWeb's database: Canada, United States
U.S. state distribution from AmphibiaWeb's database: Alabama, Arkansas, Connecticut, Delaware, Georgia, Illinois, Indiana, Kentucky, Louisiana, Massachusetts, Maryland, Maine, Michigan, Minnesota, Missouri, Mississippi, North Carolina, New Hampshire, New Jersey, New York, Ohio, Oklahoma, Pennsylvania, Rhode Island, South Carolina, Tennessee, Texas, Virginia, Vermont, Wisconsin, West Virginia
Canadian province distribution from AmphibiaWeb's database: New Brunswick, Nova Scotia, Ontario, Prince Edward Island, Quebec
Life History, Abundance, Activity, and Special Behaviors
Breeding typically takes place en masse, where males are often known to congregate earlier at the breeding pond. The sex ratio at the breeding pond is often skewed in favor of males by 1.5-3.5 times more. Husting (1965) had shown a ratio of 4.43 males per female after a 4-year study. Beginning breeding times vary geographically, though generally in the southern portion of the range breeding begins as early as December, and in the more northern portions of the range March-April. Length of the breeding season varies significantly with location and may range from 3 days to over two months. Typically, the more northern populations have two or three highly synchronized breeding bouts, often only lasting 2-3 days (Petranka 1998).
A fairly elaborate courtship may take place at breeding in which the male contacts a female and engages in a nudging ritual. After the male repeatedly encircles the female, he deposits spermatophores on the substrate for the female to pick up with her cloaca. Males will often deposit their spermatophores on top of other males' spermatophores if encountered during the courtship. The female deposits the egg masses within 2-3 days after fertilization, attaching them to submerged vegetation. The embryonic period typically lasts between 4-7 weeks, and larvae metamorphose after 2-4 months. In some cases slow growing larvae may not transform until the following spring or summer, overwintering in the pond. Within a few weeks after metamorphosis, the newly transformed salamanders disperse into the surrounding upland habitat during moist weather. It is not clear how long the juvenile stage lasts, though time to reproductive maturity is believed to be 2-3 years (slightly longer for females), at which time individuals return to the pool to breed (Petranka 1998).
Despite the relative isolation of suitable breeding sites and the high tendency towards site fidelity, migration between ponds does occur. Zamudio and Wieczorek (2007) found that immigration between ponds was common within demes in their Tompkins County, New York study populations. Their data suggested that A. maculatum breeding groups were behaving as metapopulations, such that population clusters were the functional units but with sufficient migration between demes to enable potential rescue and recolonization. High gene flow was also found between A. maculatum breeding ponds in northeastern Ohio, despite landscape fragmentation (Purrenhage et al. 2009).
Spotted salamander egg masses are preyed on by wood frog tadpoles (Rana sylvatica) as the embryos near the end of development; in turn, the frog tadpoles are preyed on by larval salamanders (Petranka et al. 1998).
This salamander is the first vertebrate reported to have photosynthetic symbionts within its cells and tissues, the single-celled alga Oophila amblystomatis. Previously it had been thought that the algae were external to the salamander embryos, but recent work by Ryan Kerney of the University of Dalhousie shows that the algae are actually within the embryonic cells. The symbiosis does not last through development; algal cells are detectable up through larval stage 44, and fewer algal cells are present in later stage larvae (Kerney et al. 2011). Transmission may take place in the salamander oviduct (Kerney et al. 2011). For a commentary on the initial report of this work at the July 2010 Ninth Internal Congress of Vertebrate Morphology (held in Uruguay) and a photo of the algae-harboring embryos, see Petherick (2010) in Nature News.
Trends and Threats
Timber harvesting significantly changes the habitat by reducing forest floor litter (decayed woody debris), understory vegetation, and canopy closure in areas surrounding breeding sites (deMaynadier and Hunter 1999). These changes affect not only the immediately impacted forest area but also affect whether habitat is suitable for salamanders in surrounding uncut forest, at least 25-35 m in (deMaynadier and Hunter 1998).
If roads run near breeding sites, salamanders may be crushed by cars. Roads may also serve as a partial barrier to movement, further fragmenting the habitat (deMaynadier and Hunter 2000).
In addition to habitat loss and fragmentation this species (especially at the larval stage) may be sensitive to decreased pH levels in breeding pools due to increased acid deposition from weather patterns or road salting (Turtle 2000; Sadinski and Dunson 1992). Low pH (<6.0) has a deleterious effect on embryo survival, although reproduction can occur in ponds with low pH (Cook 1983; Blem and Blem 1989).
Pesticide contamination of water can significantly impact A. maculatum. Low-level contamination of ponds with pyrethroid insecticides (permethrin and fenvalerate, administered at .01 to 2 ppm, singly or in combination, for 22 or 96 hours) did not kill spotted salamander larvae but was found to affect behavior. Larvae that were prodded responded abnormally by twisting rather than swimming away, suggesting that a single episode of pesticide exposure was enough to render them more vulnerable to predation. Of the five amphibian species tested (four anurans plus A. maculatum), A. maculatum was most susceptible to pesticide effects (Berrill et al. 1993). Mesocosms treated with the insecticide carbaryl (at 3.5 or 7.0 mg/ml) had high mortality of spotted salamander embryos (Boone and James 2003).
It is not known whether chytrid infection leads to mortality in this species, but it can be infected with chytrid; Ouellet et al. (2005) found evidence of Bd infection in 4 of 139 Canadian specimens.
Possible reasons for amphibian decline
General habitat alteration and loss
Berrill, M., Bertram, S., Wilson, A., Louis, S., Brigham, D., Stromberg, C. (1993). ''Lethal and sublethal impacts of pyrethroid insecticides on amphibian embryos and tadpoles.'' Environmental Toxicology and Chemistry, 12, 525-539.
Blem, C. R. and Blem, L. B. (1989). ''Tolerance of acidity in a Virginia population of the spotted salamander, Ambystoma maculatum, (Amphibia: Ambystomatidae).'' Brimleyana, 17, 37-45.
Boone, M. D., and James, S. M. (2003). ''Interactions of an insecticide, herbicide, and natural stressors in amphibian community mesocosms.'' Ecological Applications, 13, 829-841.
Cook, R. P. (1983). ''Effects of acid precipitation on embryonic mortality of Ambystoma salamanders in the Connecticut Valley of Massachusetts.'' Biological Conservation, 27, 77-88.
Husting, E.L. (1965). ''Survival and breeding structure in a population of Ambystoma maculatum.'' Copeia, 1965(3), 352-362.
Kerney, R., Kim, E., Hangarter, R. P., Heiss, A. A., Bishop, C. D., and Hall, B. K. (2011). ''Intracellular invasion of green algae in a salamander host.'' Proceedings of the National Academy of Sciences of the United States of America, Published online before print, April 4, 2011( doi: 10.1073/pnas.1018259108 ).
Ouellet, M., Mikaelian, I., Paul, B. D., Rodrigue, J., and Green, D. M. (2005). ''Historical evidence of widespread chytrid infection in North American amphibian populations.'' Conservation Biology, 19, 1431-1440.
Petherick, A. (2010). ''A solar salamander.'' Nature News, doi:10.1038/news.2010.384.
Petranka, J. W. (1998). Salamanders of the United States and Canada. Smithsonian Institution Press, Washington and London.
Petranka, J. W., Rushlow, A. W., and Hopey, M. E. (1998). ''Predation by tadpoles of Rana sylvatica on embryos of Ambystoma maculatum: implications of ecological role reversals by Rana (predator) and Ambystoma (prey).'' Herpetologica, 54, 1-13.
Phillips, C.A. (1994). ''Geographic distribution of mtDNA variants and the historical biogeography of the spotted salamander, Ambystoma maculatum.'' Evolution, 48, 597-607.
Purrenhage, J. L., Niewiaroski, P. H., and Moore, F. B.-G. (2009). ''Population structure of spotted salamanders (Ambystoma maculatum) in a fragmented landscape.'' Molecular Ecology, 18, 235-247.
Sadinski, W. J. and Dunson, W. A. (1992). ''A multilevel study of effects of low pH on amphibians of temporary ponds.'' Journal of Herpetology, 26, 413-422.
Shoop, C.R. (1994). ''Migratory orientation of Ambystoma maculatum: movements near breeding ponds and displacements of migrating individuals.'' The Biological Bulletin, 135, 230-238.
Whitford, W. G., and Vinegar, A. (1966). ''Homing, survivorship, and overwintering larvae in Spotted Salamanders, Ambystoma maculatum.'' Copeia, 1966, 515-519.
Zamudio, K. R., and Wieczorek, A. M. (2007). ''Fine-scale spatial genetic structure and dispersal among spotted salamander ( Ambystoma maculatum ) breeding populations.'' Molecular Ecology, 16, 257-274.
deMaynadier, P. G. and Hunter, M. L. Jr. (1998). ''Effects of silvicultural edges on the distribution and abundance of amphibians in Maine.'' Conservation Biology, 12, 340-352.
deMaynadier, P. G. and Hunter, M. L. Jr. (1999). ''Forest canopy closure and juvenile emigration by pool-breeding amphibians in Maine.'' Journal of Wildlife Management, 63, 441-450.
Written by Mark D. Cooperman (mark.cooperman AT tufts.edu), Tufts University
First submitted 2003-01-09
Edited by Meredith Mahoney, Kellie Whittaker (2011-04-11)
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